We leverage single particle cryoEM under defined atmospheric environments to gain atomic views of nitrogenase in action, facilitating new insights into the atomic basis for its enzymatic mechanism.

We combine cryoET, mass spectrometry and single particle cryoEM, to gain unique, in situ views of biological nitrogen fixation.

We apply cryoET to organisms grown under carefully controlled micro- and anaerobic environments to probe microbial mechanisms for survival in variably oxygenated environments.